Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout.
نویسندگان
چکیده
A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues.
منابع مشابه
Detection and identification of virulent Yersinia ruckeri: the causative agent of enteric redmouth disease in rainbow trout (Oncorhynchus mykiss) cultured in Fars province, Iran
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 65 1 شماره
صفحات -
تاریخ انتشار 1999